Koyun alveoler makrofajlarında Mannheimia haemolytica'ya yanıt olarak bağışıklık ile ilişkili genlerin in vitro mRNA ekspresyon dinamikleri

Alveolar macrophages (AMs) respond to these infections as the first line of defense and trigger lung's immune response. Knowing expression dynamics key immune-related genes in alveolar against M. haemolytica challenge will help deepen understanding disease immunopathogenesis. For this purpose, time an vitro experimental model infection was used investigate pattern Interleukin-1β, IL-6, IL-8, IL-10, NF-κB a dose time-dependent manner. A total 95% pure culture plates were obtained from bronchoalveolar lavage (BAL) fluid treated with three doses (T1: 1800, T2: 2700, T3: 5400 CFU) inoculum, while keeping one untreated control. Then, RNA extracted, cDNA synthesized. The real-time quantification data indicated that IL-1β, significantly changed compared Our study revealed exposure stimulates response sheep